neuronal class iii β-tubulin (tuj1) primary antibody d71g9  (Cell Signaling Technology Inc)

Journal: Materials Today Bio

Article Title: Dorsal root ganglion-targeted analgesic delivery for effective relief of neuropathic pain

doi: 10.1016/j.mtbio.2025.102025

Figure Lengend Snippet: In vivo DRG-targeting ability of BK-LNPs in SNI and CINP. (A) t-distributed stochastic neighbor embedding (t-SNE) visualization of Adam8 mRNA expression patterns across DRG cellular subpopulations and (B) RNAscope imaging for Adam8 mRNA in the DRG neurons following SNI modeling. (C–D) Relative Adam8 mRNA expression level in the DRG on the 7th and 28th days following SNI modeling. (E) CLSM images of the bilateral DRGs ipsilateral and contralateral to SNI surgical site and (F) corresponding quantitative analysis of RhB fluorescence. Tuj1 was a neuronal marker. (G) Schematic illustration for the bilateral DRGs ipsilateral and contralateral to SNI surgical site. (H) Relative Adam8 mRNA expression level in the DRG as well as in the heart, liver, spleen, lung, and kidney on the 7th day following CINP modeling. (I) CLSM images of the DRG in CINP and (J) corresponding quantitative analysis of RhB fluorescence. For CLSM imaging, mice were intravenously injected with LNP/RhB and BK-LNPs/RhB and at 6 h post-injection, mice were euthanized for analysis. Data were expressed as mean ± SEM (n = 3 for C and D; n = 5 for F, H, and J). ∗∗ p < 0.01 and ∗∗∗ p < 0.001.

Article Snippet: The obtained DRG sections (14 μm in thickness) were blocked with 10 % BSA for 1 h and then sequentially incubated with neuronal class III β-tubulin (Tuj1) primary antibody (D71G9, rabbit, Cell Signaling Technology, #5568, 1:1000) overnight and its fluorescence-labeled secondary antibody (Alexa Fluor 488 anti-rabbit IgG, donkey, ThermoFisher, #A-21206, 1:500) for 2 h. The fluorescence of Tuj1 and RhB was captured on the SP8 CLSM using the corresponding filters to evaluate the targeting ability of BK-1361.

Techniques: In Vivo, Expressing, RNAscope, Imaging, Fluorescence, Marker, Injection

Journal: eLife

Article Title: Regulative synthesis of capsular polysaccharides in the pathogenesis of Streptococcus suis

doi: 10.7554/eLife.101760

Figure Lengend Snippet: Mice were inoculated with acetic acid through the nostril, and 1 hr later, infected intranasally (i.n.) with 05ZYH33. ( A ) Sagittal views of the olfactory system. Sections of the distal nasal cavity and olfactory epithelium from uninfected (a, c, and e) and 05ZYH33-infected (b, d, and f) mice. Red or orange, 05ZYH33; green, the neuronal marker β-tubulin III; blue, DNA. NC, nasal cavity; OE, olfactory epithelium; LP, lamina propria; CP, cribriform plate; OB, olfactory bulb. ( B ) A schematic drawing of the sagittal plane of the rodent nose elucidates the compartments of the olfactory bulb (OB), olfactory epithelium (OE), nasal cavity (NC), and brain. This panel is redrawn from ‘Mouse Olfactory System’ ( inspiredpencil.com ). The dotted line indicates the anteroposterior localization of the coronal sections in C. ( C ) One hour or nine days after 05ZYH33 infection, coronal brain sections were prepared and stained with hematoxylin and eosin (H&E). The sections display the regions of the ventral striatum and basal forebrain located behind the anterior olfactory nucleus. Arrows indicate infiltrated inflammatory cells in the lower areas of the ventral striatum or basal forebrain. ( D, E ) Mice were inoculated with acetic acid or phosphate-buffered saline (PBS), and 1 hr later infected i.n. with 05ZYH33 or ∆2BSS2. ( D ) Colony-forming units (CFUs) of 05ZYH33 or ( E ) ∆2BSS2 in nasal-associated lymphoid tissue (NALT), the cerebrospinal fluid (CSF), and blood were determined 1 hr after infection.

Article Snippet: Immunostaining was conducted using a primary rabbit anti- S. suis antibody (1:100) and a mouse anti-tubulin beta III antibody (Merck Millipore, Darmstadt, Germany catalog no. mab5564, 1:300) to detect neuronal structures, and then appropriate fluorophore-conjugated secondary antibodies: donkey anti-rabbit Cy3 (Jackson ImmunoResearch Laboratories, Ely, UK, catalog no. 711-166-152, 1:1000), donkey anti-mouse FITC (Jackson ImmunoResearch Laboratories, Ely, UK, catalog no. 715-096-150, 1:500).

Techniques: Infection, Marker, Staining, Saline